Assessment of the Toxicity of the Crude Extract of Hibiscus Rosa-Sinensis Linn. Leaves and the Effect of Gamma Radiation on This Extract

The study assessed the toxicity and effects of gamma radiation on the crude extract of Hibiscus rosa-sinensis leaves. It found that both non-irradiated and irradiated extracts showed significant antioxidant activity, which was enhanced by gamma radiation. The radiation reduced the concentration of flavonoids and polyphenols, as well as maslinic acid in the extract. Acute and subacute toxicity tests on Wistar rats showed no behavioral changes, toxicity signs, or mortality, and there were no significant changes in body weight, organ weight, or hematological and biochemical parameters, indicating no hepato or nephrotoxicity. The study suggested that gamma radiation could enhance the therapeutic properties of the plant without introducing toxicity. Further research was recommended to explore long-term effects and clarify biochemical variations.