Topological Markers for Subcellular Chemical Mapping Using TOF-SIMS
January 2019
in “
Florida International University Digital Commons (Florida International University)
”
Studysummary This research describes using advanced mass spectrometry imaging techniques to analyze gunshot residue and map molecular components in biological samples, such as tumors and mosquito ovarian follicles, at unprecedented spatial resolution and specificity.
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The study utilized Time-of-Flight Secondary Ion Mass Spectrometry (TOF-SIMS) to enhance the specificity and reliability of sub-cellular chemical mapping through the use of endogenous chemical species, three-dimensional dual-beam depth profiling, and multi-modal mass spectrometry approaches. This technique allowed for the confirmation of gunshot residue origin and the localization and quantification of anti-tumor drugs in glioblastoma cells. Additionally, it enabled the identification of molecular components in mosquito ovarian follicles and the mapping of de novo synthesized triacylglycerides in mosquitoes. A novel multi-modal imaging protocol was also developed for characterizing proteins and small molecules in human acute wound healing.
