Decision Letter: Plant Trans-Golgi Network/Early Endosome pH Regulation Requires Cation Chloride Cotransporter (CCC1)

The study explored the role of the Cation Chloride Cotransporter (CCC1) in regulating pH and ion homeostasis at the Trans-Golgi Network/Early Endosome (TGN/EE) in Arabidopsis thaliana. CCC1 was found to be crucial for maintaining ion balance, with its absence leading to defects in endomembrane trafficking and root hair cell elongation, which could be rescued by external osmolytes like mannitol. The research faced criticism for lacking data on the functionality of the GFP-CCC1 construct and its role in endocytosis, leading to the paper not being accepted for publication. Despite these issues, the study highlighted CCC1's importance in plant cellular processes and suggested further experiments to confirm its localization and broader role in endomembrane compartments.