Author Response: Plant Trans-Golgi Network/Early Endosome pH Regulation Requires Cation Chloride Cotransporter (CCC1)
October 2021
Studysummary This study identified the Arabidopsis cation chloride cotransporter CCC1 as essential for regulating pH and function in the trans-Golgi network/early endosome, with its absence causing significant growth and stress response defects.
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The study investigated the role of the Cation Chloride Cotransporter (CCC1) in regulating ion homeostasis and pH at the Trans-Golgi Network/Early Endosome (TGN/EE) in Arabidopsis thaliana. CCC1 was found to be crucial for normal endomembrane trafficking, with its absence leading to defects in root hair cell elongation and patterning, which could be mitigated by external osmolytes like mannitol. The research highlighted CCC1's importance in maintaining endosomal pH balance alongside other transporters. Despite challenges in demonstrating the subcellular localization of CCC1 and the functionality of the GFP-CCC1 fusion protein, the study provided evidence of CCC1's role in ion control and pH regulation. The authors addressed reviewer concerns by providing additional data and clarifications, although the paper was not accepted for publication due to these issues.
