Sulfated Hyaluronan-Containing Artificial Extracellular Matrices Promote Proliferation of Keratinocytes and Melanotic Phenotype of Melanocytes from the Outer Root Sheath of Hair Follicles

March 2019 in “ Journal of Biomedical Materials Research Part A ”

M. Schneider, Sandra Rother, Stephanie Möller, Matthias Schnabelrauch, Dieter Scharnweber, Jan‐Christoph Simon, Vera Hintze, Vuk Savković

sulfated hyaluronan artificial extracellular matrix keratinocytes melanocytes outer root sheath hair follicles collagen CD44 receptor epidermal grafts

Studysummary This laboratory study found that collagen matrices with high-sulfated hyaluronan may enhance the cultivation of human keratinocytes and melanocytes from hair follicles for epidermal graft development.

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The study aimed to improve cultivation conditions for human keratinocytes (HUKORS) and melanocytes (HUMORS) from the outer root sheath of human hair follicles to generate epidermal grafts. Researchers used artificial extracellular matrix coatings made of collagen and hyaluronan with varying sulfation degrees. The results indicated that these matrices, particularly those with high-sulfated hyaluronan, provided a favorable environment for keratinocyte proliferation and enhanced the melanotic phenotype in melanocytes. This was evidenced by increased gene expression of microphthalmia-associated transcription factor and other melanotic genes. The study suggested that sulfated hyaluronan might enhance the melanotic phenotype by binding to the CD44 receptor or concentrating signaling mediators. These findings highlighted the potential of collagen matrices with sulfated hyaluronan to significantly improve the development of outer root sheath-based epidermal grafts.

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research A Human Serum-Enriched Medium Formulation Supports High Viability and Marker Expression in Primary Melanocyte Cultures from the Outer Root Sheath and Epidermis

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This study developed a new melanocyte cultivation medium without chemical mitogens, melanogenesis enhancers, or bovine products, demonstrating successful proliferation and melanotic differentiation of human melanocytes from hair follicles.

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7 citations , July 2016 in “Journal of Biomedical Materials Research Part A”

This study found that a novel hydrogel scaffold, cGEL, significantly increased melanin production and melanotic gene expression in human melanocytes compared to other graft materials, suggesting its potential as a superior carrier for skin grafting.

research Melanocytes from the Outer Root Sheath of Hair Follicles Cultivated on Collagen Membrane Improve Their Melanotic Properties

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In this study, collagen I decellularized scaffolds enhanced the growth and melanotic properties of hair follicle melanocytes, suggesting their potential as effective carriers for transplantation in vitiligo treatment.