Effects of Storage Culture Media, Temperature, and Duration on Human Adipose-Derived Stem Cell Viability for Clinical Use
January 2019
in “
Molecular Medicine Reports
”
New to Platelet-Rich Plasma? There is a guide in the encyclopedia. Read the guide → Studysummary This study found that for optimal ADSC viability during short-term storage, using 10% human serum at 4°C and utilizing the cells within 2 hours is most effective.
Our plain-language summary. Not medical advice or a treatment recommendation. Consult a qualified healthcare professional before changing treatment. Full disclaimer
The study investigated the optimal conditions for short-term storage of human adipose-derived stem cells (ADSCs) to maintain their viability for clinical use. It found that storing ADSCs at 4°C in 10% human serum (HS) was the most effective method, providing the highest survival rate and avoiding the need for cryoprotectants, which can cause toxicity. This method outperformed storage in 10% platelet-rich plasma (PRP) and 0.9% NaCl. The study emphasized that ADSCs should be used within 2 to 4 hours of storage to ensure their functionality, highlighting the importance of standardizing ADSC preparation and storage for clinical applications.
