Stereological Quantification of Cell-Cycle Kinetics and Mobilization of Epithelial Stem Cells During Wound Healing
January 2016
in “
Methods in molecular biology
”
TLDR The method helps estimate and track skin cell growth and movement during healing.
The study described a stereology method to estimate the total number of proliferative and migratory epithelial cells during wound healing. By using pulse and chase experiments with halogenated thymidine analogs like iododeoxyuridine (IdU) and chlorodeoxyuridine (CldU), researchers could track epithelial cell populations with different proliferative characteristics across skin compartments. This method provided valuable insights into skin stem-cell biology and could be widely applied to address key questions in this field.
