Stereological Quantification of Cell-Cycle Kinetics and Mobilization of Epithelial Stem Cells During Wound Healing

January 2016 in “ Methods in molecular biology ”

Eduardo Martínez‐Martínez, Eileen Uribe‐Querol, Claudio Iván Galván-Hernández, Gabriel Gutiérrez‐Ospina

stereology epithelial stem cells wound healing halogenated thymidine analogs iododeoxyuridine chlorodeoxyuridine proliferative characteristics skin compartments skin stem-cell biology IdU CldU

TLDR The method helps estimate and track skin cell growth and movement during healing.

The study described a stereology method to estimate the total number of proliferative and migratory epithelial cells during wound healing. By using pulse and chase experiments with halogenated thymidine analogs like iododeoxyuridine (IdU) and chlorodeoxyuridine (CldU), researchers could track epithelial cell populations with different proliferative characteristics across skin compartments. This method provided valuable insights into skin stem-cell biology and could be widely applied to address key questions in this field.

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