Elucidation Of Stem Cell Formation Mechanism By Fibroblast Aggregates
January 2014
in “
Institutional Repositories DataBase (IRDB)
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Studysummary The study observed that cell spheres formed from mesenchymal cells of newborn mice skin exhibited upregulation of markers linked to hair follicle regeneration, suggesting a potential hibernation-like state for maintaining viability.
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The study investigated the formation of stem cells using fibroblast spheres derived from mesenchymal cells extracted from the skin of newborn C57BL/6J mice. These cell spheres were cultured in serum-free medium on non-adhesive dishes and remained viable for several months, suggesting a hibernation-like state. Genetic and immunohistological analyses revealed that markers related to hair follicle regeneration and the hair cycle, such as Wnt, as well as undifferentiated markers like Sox-2, Oct-4, CD133, and cxcr4, were upregulated in the cell spheres. However, the distribution of these markers was uneven, indicating a need for further investigation to determine whether this was due to changes in specific cell characteristics or selective cell processes.
