Single-Cell RNA Sequencing Redefines Macrophage Heterogeneity and Function in Wound Healing

This review article discusses the role of single-cell RNA sequencing (SCS) in understanding the complexity of skin wound healing, particularly focusing on macrophage heterogeneity and function. SCS is highlighted as a transformative tool that reveals the diverse roles of macrophages, which are crucial for tissue homeostasis, debris removal, extracellular matrix remodeling, and cytokine secretion during wound healing. The study identifies various macrophage subtypes, such as CD301b+ macrophages, monocyte-derived macrophages, and tissue-resident macrophages, and summarizes key marker genes involved in these processes. These insights significantly enhance the understanding of macrophage roles in wound healing at the single-cell level.