Simultaneous Determination of Selected Steroids with Neuroactive Effects in Human Serum by Ultrahigh-Performance Liquid Chromatography–Tandem Mass Spectrometry

The study developed and validated a method for the simultaneous determination of nine neuroactive steroids in human serum using ultrahigh-performance liquid chromatography with tandem mass spectrometry. This method allows for high throughput and sensitive quantitative analysis in 150 μL of serum, with linearity in the range of 0.90 nmol/L to 28.46 μmol/L and accuracy and precision ranging from 83 to 118% and 0.9 to 14.1%, respectively. The method could enhance understanding of the pathophysiology of neurological and psychiatric diseases and aid in identifying new biomarkers and therapeutic approaches.