The Utilization of Sex Hormone Antibodies for Screening and Separation of Trace Biological Mixtures

January 2020 in “ VCU Scholars Compass (Virginia Commonwealth University) ”

Kristin N. Jones

sex hormone antibodies testosterone dihydrotestosterone estradiol epithelial skin cells anti-steroid antibodies DHT

TLDR Sex hormone antibodies can help identify male and female contributors in forensic samples.

This study explored the use of sex hormone antibodies to differentiate trace biological mixtures, specifically focusing on epithelial skin cells, which are a common type of forensic evidence. By using antibodies targeting testosterone, dihydrotestosterone, and estradiol, researchers aimed to optimize antibody staining to distinguish between male and female contributors. The study found that while not all individuals could be differentiated, improved signal detection was possible, as shown by increased median fluorescence and separation in flow cytometry histograms. This led to a protocol for labeling contributors with anti-steroid antibodies, potentially simplifying complex trace mixtures for DNA profiling in forensic casework.

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