Effects of Rosemary Oil on Enzyme Activities and Cytokine Expression Related to Hair Growth in C57BL/6 Mice

This study investigated the effects of rosemary oil on enzyme activities and cytokine expression related to hair growth in C57BL/6 mice. Six-week-old male mice were divided into four groups: normal (saline), vehicle control (jojoba oil), positive control (3% minoxidil), and experimental (3% rosemary oil). The treatments were applied topically for 4 weeks. Results showed that dermal ALP and γ-GT activities, as well as IGF-1 mRNA expression, were significantly increased in both the positive control and experimental groups compared to the normal group (p<0.05). SCF antigens were heavily stained in the bulge and sebaceous gland in the experimental and positive control groups. These findings suggested that rosemary oil promoted ALP and γ-GT activities and IGF-1 mRNA expression in the skin tissues of C57BL/6 mice.