Decoding The Role Of OsPRX83 In Enhancing Osmotic Stress Tolerance In Rice Through ABA-Dependent Pathways And ROS Scavenging

The study explores the role of the OsPRX83 gene in rice, demonstrating its importance in enhancing osmotic stress tolerance through ABA-dependent pathways and ROS scavenging. Researchers used overexpression and CRISPR-Cas9 mutant lines to show that OsPRX83 expression is induced by stressors such as H2O2, PEG, NaCl, and ABA. Overexpression of OsPRX83 resulted in improved osmotic and oxidative stress tolerance, with higher survival rates and increased peroxidase activities compared to wild-type and mutant plants. The OE-12 line, for instance, showed an 87% survival rate under osmotic stress, compared to 60% in wild-type and 30% in mutant lines. Transcriptome analysis indicated that OsPRX83 modulates the expression of genes involved in ROS scavenging, suggesting its crucial role in stress tolerance and potential for developing drought-resistant rice varieties.