Interaction of OsRopGEF3 Protein With OsRac3 to Regulate Root Hair Elongation and Reactive Oxygen Species Formation in Rice

The study investigated the role of the OsRopGEF3 protein in rice root hair growth and reactive oxygen species (ROS) formation. Researchers used CRISPR-Cas9 to create loss-of-function mutants of OsRopGEF3, which resulted in reduced root hair length and increased width, along with decreased ROS levels. The study found that OsRopGEF3 interacts with OsRac3, a protein involved in root hair elongation, and this interaction was confirmed through various assays. Additionally, OsRac3 was shown to interact with OsRBOH5, a gene involved in ROS production. The findings suggested that OsRopGEF3 plays a crucial role in converting GDP to GTP in OsRac3, which then interacts with OsRBOH5 to produce ROS, highlighting OsRopGEF3 as a key regulator of root hair growth in rice.