Restructuring skin equivalents with human hair follicle orscs and fibroblasts

March 2007 in “ Journal of Plastic Reconstructive & Aesthetic Surgery ”

Shanshan Liu, T. Egana, I. Wilcke, Juergen Lohmeyer, Daniel Thomé, Enikő Bodó, Zhongfa Lu, Stefan Krüger, P. Mailänder, Hans-Günther Machens

TLDR A new method was developed to create better skin models for healing and reconstruction.

The study focused on developing a three-dimensional skin equivalent (SE) model using human dermal fibroblasts, a collagen-GAG matrix, and outer root sheath cells (ORSCs) from hair follicles. The bulge region of the hair follicle, which contains multipotent stem cells, was utilized to improve the quality of the SE. This approach aimed to create a rapid, easy, and effective method for producing skin equivalents, potentially benefiting wound healing and skin reconstruction applications.

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Research cited in this study

10 / 10 results

research Characterization and Isolation of Stem Cell-Enriched Human Hair Follicle Bulge Cells

550 citations , December 2005 in “The Journal of clinical investigation/The journal of clinical investigation”

Researchers successfully isolated and identified key markers of stem cell-enriched human hair follicle bulge cells.

research A Review: The Location, Molecular Characterisation and Multipotency of Hair Follicle Epidermal Stem Cells

61 citations , November 2004 in “Annals of the Academy of Medicine Singapore”

Hair follicle stem cells are mainly in the bulge region and can help repair skin and form hair and glands, but more research is needed to fully understand them.

research Human Follicular Stem Cells: Their Presence in Plucked Hair and Follicular Cell Culture

82 citations , May 2004 in “British Journal of Dermatology”

Stem cells are present in plucked hair follicles and can be maintained in culture.

research An Improved and Rapid Method to Construct Skin Equivalents from Human Hair Follicles and Fibroblasts

48 citations , August 2001 in “Experimental dermatology”

Researchers created a quick, cost-effective way to make skin-like tissue from hair follicles and fibroblasts.

research Involvement of Follicular Stem Cells in Forming Not Only the Follicle but Also the Epidermis

1010 citations , August 2000 in “Cell”

Hair follicle stem cells can form both hair follicles and skin.

research Characterization of a new tissue-engineered human skin equivalent with hair

221 citations , June 1999 in “In Vitro Cellular & Developmental Biology - Animal”

research Successful Treatment of Chronic Leg Ulcers with Epidermal Equivalents Generated from Cultured Autologous Outer Root Sheath Cells

99 citations , July 1996 in “Journal of Investigative Dermatology”

research Keratin 19 as a biochemical marker of skin stem cells in vivo and in vitro: keratin 19 expressing cells are differentially localized in function of anatomic sites, and their number varies with donor age and culture stage

441 citations , May 1996 in “Journal of Cell Science”

Keratin 19 helps identify skin stem cells, with its presence varying by body location, age, and culture stage.

research Architecture of Reconstructed Epidermis on Collagen Lattices Varies according to the Method Used: A Comparative Study

9 citations , January 1990 in “Skin Pharmacology and Physiology”

The method and source of keratinocytes affect the structure of reconstructed skin.

research Keratin 19: Predicted Amino Acid Sequence and Broad Tissue Distribution Suggest it Evolved from Keratinocyte Keratins.

238 citations , May 1989 in “Journal of Investigative Dermatology”

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research Expression of E6 and E7 papillomavirus oncogenes in the outer root sheath of hair follicles extends the growth phase and bypasses resting at telogen.

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research Outer root sheath keratinization in anagen and catagen of the mammalian hair follicle. A seventh distinct type of keratinization in the hair follicle: trichilemmal keratinization.

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Trichilemmal keratinization is a unique process in hair follicles where the outer root sheath turns into keratin without a specific layer.