Rapid Preparation of a Noncultured Skin Cell Suspension That Promotes Wound Healing

In the 2017 study, researchers successfully developed a method to prepare a noncultured autologous skin cell suspension (NCSCs) using a tissue homogenizer, which enhanced wound healing in a nude-rat burn model. The optimal homogenization conditions were determined, resulting in cell viability between 52 to 89% and the presence of keratinocyte-like cells and growth factors in the NCSCs. The application of NCSCs to burn wounds in rats led to significantly improved skin regeneration and adnexa regeneration by day 14 compared to a control group, indicating the potential of this method as a low-cost and effective treatment for burns. However, the study noted limitations such as not confirming the true viability of cells in the NCSCs and the need for further research to understand the mechanisms behind the wound healing effects. No significant conflicts of interest were reported.