Retinoic Acid And Dimethyl Sulfoxide Promote Efficient Delivery Of Transgenes To Mouse Skin By Topically Transdermal Penetration

The study investigated a method for gene therapy in mouse skin using retinoic acid (RA) and dimethyl sulfoxide (DMSO) to enhance the transdermal delivery of plasmid DNA. It was found that RA significantly improved gene transfection efficiency in the epidermis and hair follicle cells, while a 10% concentration of DMSO was optimal for minimizing side effects. This approach was less toxic and more cost-effective compared to traditional carriers like liposomes. The method showed successful localized transfection without detectable DNA in the blood, suggesting its potential for treating skin and hair disorders through topical gene therapy, although further research was needed for pre-clinical validation.