Protection Against Cyclophosphamide-Induced Alopecia and Inhibition of Mammary Tumor Growth by Topical 1,25-Dihydroxyvitamin D3 in Mice

The study investigated the effects of topical 1,25-dihydroxyvitamin D3 (1,25(OH)2D3) on cyclophosphamide (CTX)-induced alopecia and mammary tumor growth in 21-day-old BALB/c mice. Mice treated with 1,25(OH)2D3 showed significant protection against CTX-induced hair loss, with a more pronounced effect in female mice inoculated with EMT-6 mammary tumors. Additionally, 1,25(OH)2D3 alone inhibited tumor growth in both male and female mice and enhanced the anti-tumor effects of CTX. The results demonstrated that 1,25(OH)2D3 could protect against CTX-induced alopecia and inhibit tumor growth, with varying effects based on sex and tumor presence.