The Effect of Oxidized Low-Density Lipoprotein Compared to Cytokine-Induced Inflammation on the Differentiation Properties of Human Gingival Mesenchymal Stem Cells
TLDR Oxidized LDL reduces cell growth but affects stem cell differentiation less negatively than cytokine-induced inflammation.
This study investigated the effects of oxidized low-density lipoprotein (oxLDL) compared to cytokine-induced inflammation on human gingival mesenchymal stem cells (G-MSCs). Over 7 days, G-MSCs were exposed to a control, cytokine mixture, or oxLDL. Results showed that while initial cell proliferation increased with inflammatory stimuli, cell numbers were highest in the control group after 14 days, followed by the cytokine group, and lowest in the oxLDL group. Osteogenic differentiation was significantly lower in the cytokine group compared to oxLDL, with minor differences in chondrogenic and adipogenic differentiation. G-MSCs responded differently to oxLDL-induced metabolic inflammation than to cytokine-mediated inflammation, showing less negative impact on differentiation but significantly reduced cell proliferation.