Optimization of Gelatin and Crosslinker Concentrations in a Gelatin/Alginate-Based Bioink with Potential Applications in a Simplified Skin Model

This study focused on developing a gelatin-based bioink for 3D bioprinting a simplified skin model. By varying gelatin (10, 15, and 20%) and calcium chloride (100, 150, and 200 mM) concentrations, the researchers found that a formulation with 15% gelatin and 150 mM CaCl₂ provided optimal printability and cell viability. This combination resulted in a homogeneous distribution of viable human fibroblasts and keratinocytes over 14 days of in vitro culture. The bioink demonstrated potential for applications in pro-reparative molecule bioactivity and drug evaluation.