Methods for Optimized Cultivation of Hair Cells From C3H Mice

October 1989 in “ PubMed ”

Nobutaka Tanigaki, Satoaki Nakamura, H Kanehisa, Yosuke Masamoto, Yukio Kitano

hair cells C3H mice dispase collagenase hair roots cell suspension serum concentrations collagen coating MCDB 153 medium dermal fibroblasts bovine pituitary extract cytoskeletal proteins

TLDR Optimized conditions and specific treatments enhance the growth of hair cells from C3H mice.

The study described methods for optimizing the cultivation of hair cells from 4-day-old C3H mice. The process involved treating the skin with dispase and collagenase to isolate hair roots, which were then dispersed into a cell suspension. The attachment and growth of hair cells were enhanced by serum concentrations, collagen coating, and the use of MCDB 153 medium, which favored hair cell growth over dermal fibroblasts. A crude bovine pituitary extract significantly boosted hair cell growth. The cultured hair cells maintained functional integrity, expressing hair-specific cytoskeletal proteins, thus providing a model system for studying hair growth.

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research Methods for Optimized Cultivation of Hair Cells From C3H Mice

6 citations , October 1989 in “PubMed”

Optimized conditions and specific treatments enhance the growth of hair cells from C3H mice.