A Nonredundant Human Protein Chip for Antibody Screening and Serum Profiling

September 2003 in “ Molecular & cellular proteomics ”

Angelika Lueking, Alexandra Possling, Otmar Huber, Allan Beveridge, Martin Horn, Holger Eickhoff, Johannes Schuchardt, Hans Lehrach, Dolores Cahill

alopecia autoimmune diseases antibody screening serum profiling protein microarray autoantigens p69 tumor suppressor p33 ING hair loss immune system diseases antibody testing blood serum analysis protein chip antigens p69 protein p33 ING protein

TLDR The technology can help diagnose and subtype autoimmune diseases by identifying specific autoantibodies.

The study developed a high-density protein microarray with 2,413 nonredundant purified human proteins for antibody screening and serum profiling. It demonstrated applications in characterizing antibody binding and profiling the antibody repertoire in sera from patients with autoimmune diseases, including alopecia and arthritis. The protein chips showed high sensitivity and specificity, identifying specific autoantigens like p69 and tumor suppressor p33 ING in alopecia patients' sera. This technology offered advantages such as reduced time, small sample volumes, and suitability for automation, suggesting potential diagnostic applications for autoimmune diseases and hair loss conditions.

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