Impact of Different Mating and Surgical Protocols on the Establishment of a Mouse Model for Fetal Scarless Skin Healing

The study refines a mouse model for fetal scarless skin healing by modifying traditional surgical protocols. Key changes include using a 19G needle instead of sutures, which reduces operative time by over 80%, decreases abortion rates, and improves pregnancy success through hormone-induced superovulation. The modified protocol allows for earlier modeling at embryonic days 15.5 and 16.5, where wounds heal with regenerated hair follicles and organized collagen, contrasting with fibrotic scars at day 18.5. This approach enhances experimental efficiency and reproducibility, providing a reliable model for studying regenerative and anti-fibrotic therapies.