Establishing Competent Morphogenetic Fields for Tissue Pattern Regeneration in Skin Organoids

The study explores the principles governing skin organoids' ability to regenerate hair by forming competent morphogenetic units (CMUs) through epidermal-dermal coupled cysts. Using scRNA-sequencing, the research identifies the emergence of new cell types and interactions during CMU formation. Key signaling pathways, such as IFNr-PKR-PKC and Dermal-Tgfb-Gsk3, are crucial for establishing basement membranes and cell polarization. While adult cells typically cannot form organoids, they can be induced to generate CMUs and regenerate hair with IFNr or VEGF. The study compares the mechanisms of morphogenetic competency in developing skin, wound-induced hair neogenesis, and organoids, highlighting both similarities and differences.