Long-Term Culture of Skin Biopsies: Maintenance of Fibroblast Production and Competency of Reprogramming

    December 2025 in “ FEBS Open Bio ”
    Sudiksha Rathan‐Kumar, Michael Ripperger, Grant Westlake, Kevin C. Ess
    Studysummary In this study, fibroblasts from long-term skin biopsy cultures retained their ability to reprogram into induced pluripotent stem cells after 16 months, despite undergoing transcriptional changes and decreased proliferation rates over successive generations.
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    Research cited in this study 2

    1. Fibroblasts: Origins, Definitions, and Functions in Health and Disease Cell · 2021
    2. Distinct Fibroblast Lineages Determine Dermal Architecture in Skin Development and Repair Nature · 2013