Reproducibility of High-Resolution Laser Speckle Contrast Imaging to Assess Cutaneous Microcirculation for Wound Healing Monitoring in Mice

The study evaluated the reproducibility of high-resolution laser speckle contrast imaging (LSCI-HR) and LSCI with oximetry (LSCI-OX) in assessing cutaneous blood flow during wound healing in mice, comparing them to laser Doppler imaging (LDI). Conducted on 16 male C57Bl/6J mice, the research found that LSCI-HR and LSCI-OX demonstrated better inter-day and inter-site reproducibility of blood perfusion measurements than LDI, with coefficients of variation (CVs) between 12% and 17% compared to LDI's 26% to 29%. However, the reproducibility of hemoglobin oxygenation with LSCI-OX was poor. The study concluded that LSCI-HR devices provided good reproducibility for assessing skin perfusion in mice, suggesting their potential utility in wound healing monitoring.