Isolate and Culture Follicular Epithelial Cells from Rat Vibrissa Follicles by Two-Step Digestion Treatment
January 2006
in “
Zhongguo bijiao yixue zazhi
”
Studysummary This study demonstrated a simple and efficient method to isolate epithelial cells from rat vibrissa follicles, reducing labor and contamination risk while maintaining positive K19 staining.
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The study developed a simple and efficient two-step digestion method for isolating and culturing epithelial cells from rat vibrissa follicles. The process involved digesting the follicles in 0.2% collagenase I and 0.1% trypsin, which reduced labor and contamination risk. The isolated cells were positive for keratin 19 staining, confirming successful isolation.