Effective High-Throughput Isolation of Enriched Platelets and Circulating Pro-Angiogenic Cells to Accelerate Skin-Wound Healing

April 2022 in “ Cellular and Molecular Life Sciences ”

Silvia Erratico, M. Belicchi, Mirella Meregalli, Dario Di Silvestre, Luana Tripodi, Antonella De Palma, Rebecca Jones, Emanuele Ferrari, Laura Porretti, Elena Trombetta, Giorgio R. Merlo, Pierluigi Mauri, Yvan Torrente

platelet-rich plasma pro-angiogenic cells Angio PRP re-epithelialization granulation tissue blood vessel regeneration elastin collagen fibers glutathione metabolism PRP

Studysummary This study reports that a new protocol using Angio PRP improved wound healing in mice by enhancing key healing processes and regulating inflammation, suggesting potential for clinical advancements. Our plain-language summary of this paper — not a Tressless recommendation.

The study developed a high-throughput protocol for isolating platelet-rich plasma (PRP) and pro-angiogenic cells, termed Angio PRP, to enhance skin wound healing. Angio PRP was shown to effectively promote wound closure, re-epithelialization, granulation tissue growth, and blood vessel regeneration. The treatment also improved the mechanical properties of mouse wounds by organizing elastin and collagen fibers correctly. Proteomic analysis revealed that Angio PRP upregulated the detoxification pathway of glutathione metabolism, which was associated with reduced inflammation. These findings provided a framework for Angio PRP's role in wound healing and suggested potential for future clinical applications.

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