Effect of Heat Treatment on Human Hair Keratin Film

    September 2013 in “ 日本香粧品学会誌 ”
    Toshihiro Fujii, Yuka Takashima, Shunsuke Takayama, Yumiko Ito, Tomoyuki Kawasoe
    TLDR Keratin films can measure hair heat damage.
    The study investigated the effects of heat treatment on human hair keratin films and hair samples, focusing on color change, morphology, protein solubility, and oxidative proteins. It was found that thermal treatments at temperatures above 170℃ caused the keratin films to change color from opaque white to yellow/light brown. Despite this color change, scanning electron microscopy revealed minimal structural changes even at 200℃ for 10 minutes. Protein solubility decreased with increased heating time and temperature, with a linear relationship observed between solubilized proteins and temperatures from 110 to 160℃. The keratin films showed more pronounced changes compared to hair samples. Additionally, the amount of carbonylated proteins increased in films treated at 120–180℃, indicating protein oxidation and the formation of a "Stable Structure." The study suggested that keratin films could be used as an alternative to hair samples for quantitatively evaluating hair thermal damage.
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