Characterizing Wool Keratin

    Jeanette M. Cardamone, Alberto Núñez, Rafael A. García, Mila Aldema-Ramos
    TLDR Wool keratin is reactive, biocompatible, biodegradable, and can model keratin from other sources.
    The study characterized wool keratin using alkaline hydrolysis methods, achieving keratin powder recovery rates between 68% and 82%. By treating wool with NaOH solutions, keratin amides and disulfides were converted into acids and smaller protein fragments, which were analyzed using techniques like MALDI-TOF and SEM. The presence of Type II keratin intermediate filament and microfibrillar proteins was identified, along with specific amino acid sequences suggesting potential for enzymatic self-crosslinking. FTIR spectra confirmed cysteic acid formation and disulfide linkage cleavage. The research demonstrated the potential for producing high-value keratin products efficiently, maintaining keratin's structural integrity, and opening new market potentials for wool.
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