Hair growth in vitro from histocultured skin
August 1992
in “
In vitro cellular & developmental biology
”
in vitro hair growth histocultured skin collagen sponge gels mouse skin specimens BCECF PI dyes DNA synthesis ³H thymidine labeling doxorubicin alopecia hair growth regulation hair growth stimulators hair growth inhibitors in vitro hair growth collagen sponge gels mouse skin BCECF PI dyes ³H thymidine doxorubicin hair loss hair growth regulation hair growth stimulators hair growth inhibitors
TLDR The new system can grow hair in the lab and test hair growth treatments.
The document reports on a new in vitro system for hair growth using sponge-gel supported histocultures of intact mouse skin. The system allowed for the maintenance of histologically-intact human skin specimens on collagen sponge gels for several days, with hair growth observed in mouse skin specimens maintained in histoculture for up to 10 days. The study used pieces of shaved mouse skin, with three types of sponge-gels as culture supports. The viability of cells was assessed using BCECF and PI dyes, and DNA synthesis was measured with [³H]thymidine labeling. Hair growth in vitro was found to be similar to in vivo patterns, although it slowed down after the first 2 days. The system was also tested with the chemotherapeutic drug doxorubicin, which is known to cause alopecia, and it successfully inhibited hair growth in the cultures. This suggests that the histoculture system can be used to study hair growth regulation and to test hair growth stimulators and inhibitors. The work was supported by a National Cancer Institute grant.
