Regeneration Genetics: Understanding Tissue Regeneration Mechanisms in Vertebrates

The document reviewed genetic approaches to understanding tissue regeneration in vertebrates, highlighting the use of transgenic models and genetic tools like Cre recombinase and CRISPR/Cas9. It discussed the identification of cellular sources and molecular factors involved in regeneration, noting that regenerative capacity varies among species and can diminish with age. The review emphasized the potential for future therapies to stimulate regeneration through molecular targets. It also covered advancements in live cell imaging and multicolor cell tagging, such as the Brainbow system, which provided insights into stem cell dynamics and tissue regeneration processes. The role of gene regulatory elements, particularly tissue regeneration enhancer elements (TREEs), was explored, suggesting their potential to enhance regenerative capacity in mammals. The document underscored the importance of early injury signals and the challenges faced in regeneration genetics, with expectations for advancements through improved genetic tools and methodologies.