Non-Small Cell Lung Carcinoma Spheroid Models in Agarose Microwells for Drug Response Studies

This study designed a micromold template for creating controlled-sized, multicellular tumor spheroids, finding that HEK-293 cells formed consistent spheroids with high circularity and viability after 7 days, influenced by cell seeding density and treatment conditions.

In this study, researchers developed a microfluidics-based method for creating uniform, size-controlled stem cell spheroids that enhance osteogenic differentiation when encapsulated in alginate-RGD microgels.

This study developed a new mask-free method using digital micromirror and microfluidic systems to create multicellular heterospheroids for drug screening, finding that heterospheroids exhibit higher drug resistance and combinatorial drugs are more effective than single drugs in cancer therapeutic applications.

In this study, cell membrane remodeling with a thermoresponsive boronic acid copolymer was shown to rapidly form spheroids from cancer or cardiac cell lines under standard conditions, promising advances in tissue engineering.