Fish Cell Spheroids, a Promising In Vitro Model to Mimic In Vivo Research: A Review

This review discusses the potential of fish cell spheroids as a promising in vitro model to mimic in vivo research. It highlights the advantages of 3D cell cultures, such as spheroids, over traditional monolayer cultures in replicating in vivo conditions. The application of fish cell spheroids is currently limited but expanding, with uses in eco-toxicology, immunology, drug screening, endocrinology, and metabolism studies. The review focuses on the methods for generating these spheroids and their significant contributions to research, emphasizing their potential for future studies.