Ex Vivo SIM-AFM Measurements Reveal the Spatial Correlation of Stiffness and Molecular Distributions in 3D Living Tissue

October 2024 in “ Acta Biomaterialia ”

Itsuki Shioka, Ritsuko Morita, Rei Yagasaki, Duligengaowa Wuergezhen, Tadahiro Yamashita, Hironobu Fujiwara, Satoru Okuda

atomic force microscopy structured illumination microscopy collagen enzymatic digestion epithelium mesenchyme freeze-thaw PFA glyoxal fixation AFM SIM paraformaldehyde

🚨 The study involves skin tissue and mechanical properties, which are somewhat relevant to scalp health and wound healing, but it does not directly address hair, hair loss, or related biological processes.

Studysummary This study introduced a novel method that uses upright structured illumination microscopy and atomic force microscopy to map the mechanical properties of thick living tissue slices, revealing the intricate mechanical heterogeneity of mouse skin and effects of preservation techniques on tissue stiffness.

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research Molecular and Spatial Landmarks of Early Mouse Skin Development

10 citations , August 2023 in “Developmental cell”

In this study, researchers used advanced techniques to map the early embryonic development of mouse skin, identifying diverse cell populations and uncovering complex interactions essential for skin structure, signaling, and function.