Distribution of Quantum Dot Nanoparticles That Penetrate Skin and Distinction of Combined Osmium Tetroxide in Electron Microscopic Analysis

The study investigated the penetration of quantum dot nanoparticles into the skin, revealing that these particles could penetrate the stratum corneum and reach sebocytes via hair follicles. A significant challenge was distinguishing the nanoparticles from artifacts caused by the combination of free fatty acids and osmium tetroxide during specimen preparation. By employing different sample processing techniques and comparing cross-correlation image patterns, researchers were able to accurately determine the distribution of the nanoparticles, thereby reducing errors in analysis. This method was anticipated to enhance the accuracy of nanoparticle distribution studies in biomedical research.