Cis-Element and Transcriptome-Based Screening of Root Hair-Specific Genes and Their Functional Characterization in Arabidopsis

    May 2009 in “ Plant physiology ”
    Su-Kyung Won, Yong-Ju Lee, Ha-Yeon Lee, Yoon-Kyung Heo, Misuk Cho, Hyung‐Taeg Cho
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    TLDR Researchers found 19 genes important for root hair growth in a plant called Arabidopsis.
    In the 2009 study, researchers identified 19 root hair-specific genes in Arabidopsis thaliana through a combination of in silico and experimental methods. They initially screened for genes with a root hair-specific cis-element in their promoters, then narrowed down the list by excluding non-root-specific genes and those not expressed in root hair-defective plant lines. The final list included many protein kinases and cell wall-related genes previously uncharacterized for their role in root hair development. Functional analysis using loss-of-function mutants and overexpressing transformants showed that these genes are involved in hair growth and morphogenesis, highlighting the importance of a specific cis-element in identifying cell type-specific genes.
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