Chemical and Photo-Oxidative Hair Damage Studied by Dye Diffusion and Electrophoresis
November 2003
in “
PubMed
”
chemical oxidative damage photochemical oxidative damage microspectrophotometric methods electrophoretic methods fluorescence microscopy SDS-PAGE hair proteins cosmetic treatments light exposure UV microspectrophotometry chemical damage photo damage fluorescence imaging protein analysis cosmetic hair treatments UV light damage
Studysummary This study found that chemical and photochemical oxidative processes modify the main classes of proteins in hair, confirmed through microspectrophotometric, fluorescence microscopy, and electrophoretic methods.
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The study by S. B. Ruetsch et al. used microspectrophotometric and electrophoretic methods to analyze the effects of chemical and photo-oxidative damage on hair. They mapped the diffusion of molecules in untreated and treated hair fibers using fluorescence microscopy and calculated diffusion coefficients. SDS-PAGE was employed to confirm these findings and to show how hair proteins are altered by cosmetic treatments and light exposure. UV microspectrophotometry also supported the results, providing an alternative method to evaluate photo-oxidative damage.
