Characterization of a Mouse Scube3 Reporter Line

This document describes the characterization of a mouse Scube3 reporter line, which was obtained to analyze the function of Scube3 during development. The study characterizes the expression pattern of Scube3 in mouse embryos and investigates the phenotype of homozygous Scube3m1Dge/H; tm1Dge/H mice. Scube3 is expressed in the developing teeth, tongue, vibrissae, and eye, with considerable overlap between Scube3m1Dge/H reporter expression and Shh transcription in the developing palate. Despite the prominent expression of Scube3 in the early facial processes and limb buds, the craniofacial and axial skeleton of Scube3m1Dge/H; tm1Dge/H mice was normal, and microCT analysis of the molar dentition in adult Scube3m1Dge/H; tm1Dge/H mice demonstrated normal gross morphology of the cusps and roots of these teeth when compared with wild type.