Cell Observation Method Under Near-Living Conditions by Scanning Electron Microscopy

The study explored a method using ionic liquid for preparing dermal papilla (DP) cell samples for scanning electron microscopy (SEM) to observe primary cilia under near-living conditions. This approach aimed to reduce the damage typically caused by conventional preparation methods, which involve multiple chemical steps and drying. The researchers successfully observed the cilium-like structures on 2D cultured DP cells, noting improvements in structural preservation and attachment to scaffoldings compared to traditional methods. However, they did not observe primary cilia in clumps of DP cells using the ionic liquid method. The study suggested that this method could potentially enhance the observation of cellular structures with less damage during preparation.