Negatively Charged Carbon Dot-Linked Glycyrrhizic Acid Hydrogel Promoted Hemostasis, Immunoregulation, and Re-Epithelialization of Wound Closure

The study presents an innovative hydrogel, CT@GA-gel, which combines charred Trachycarpus-derived carbon dots (CT-CDs) with glycyrrhizic acid (GA) to enhance wound healing, particularly for diabetic wounds. The negatively charged CT-CDs enable the formation of hydrogels at low GA concentrations (0.5%), improving mechanical properties and tissue adhesion for rapid hemostasis. The hydrogel also promotes immune regulation, re-epithelialization, and hair follicle growth, aiding chronic wound closure. Transcriptomics analysis shows increased gene expression related to hemostasis, cell adhesion, and extracellular matrix deposition, highlighting its potential in regenerative medicine for chronic tissue injuries.