Determination of Candesartan in Human Plasma by HPLC-MS-MS
January 2008
in “
Central South Pharmacy
”
TLDR A reliable method was developed to measure candesartan in human plasma.
The study established a reliable and accurate HPLC-ESI-MS method for determining candesartan in human plasma, using finasteride as an internal standard. The method involved a mobile phase of 0.1% formic acid-methanol-acetonitrile and extraction with an organic solvent. The standard curve was linear between 0.78 and 200 μg·L-1 with a correlation coefficient of 0.9945. The relative recovery ranged from 94.07% to 102.86%, and the relative standard deviation for both intraday and interday assays was less than 10%. The limit of quantitation was 0.78 μg·L-1, and the extraction recovery exceeded 88%. This method was suitable for pharmacokinetic studies of candesartan in humans.
