Control of Androgen Receptor Expression in Human Keratinocytes and a Reconstituted Human Epidermis Model with Selective Antisense Oligonucleotides

The study investigated the use of antisense oligonucleotides to control androgen receptor (AR) expression in human keratinocytes and a reconstituted human epidermis model. Researchers applied antisense oligonucleotides to primary human foreskin keratinocytes, non-foreskin keratinocytes from young and older female donors, and a reconstituted human epidermis. The transfection process involved a liposome-mediated system, and AR expression was measured using Western blotting. Results showed a reduction in AR expression by approximately 25% in foreskin keratinocytes and between 46% and 70% in the reconstituted epidermis after recovery periods. The study demonstrated that antisense oligonucleotides could effectively inhibit AR expression, suggesting potential for developing topical treatments targeting androgen-related skin disorders.