Improvement of Antioxidant Defenses in Keratinocytes Grown in Physioxia: Comparison of 2D and 3D Models
June 2023
in “
Oxidative Medicine and Cellular Longevity
”
New to catalase? There is a guide in the encyclopedia. Read the guide → Studysummary This study reports that keratinocytes grown under low oxygen conditions (physioxia) demonstrated reduced reactive oxygen species production and increased antioxidant enzyme activity compared to those in higher oxygen conditions, highlighting the role of oxygen in regulating skin antioxidant defenses.
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This study investigates the antioxidant status of keratinocytes grown under physioxia (1-3% O2) compared to normoxia, using both 2D and 3D models. It finds that physioxia promotes keratinocyte proliferation and results in a thinner epidermis due to slower cell differentiation. Under physioxia, keratinocytes produce less reactive oxygen species (ROS) upon stress, indicating better protection against oxidative stress. Despite lower or equivalent mRNA levels for antioxidant enzymes, there is higher activity for catalase and superoxide dismutases in physioxia. The study highlights the importance of oxygen levels and culture models in studying skin aging and suggests that physioxia conditions better mimic the in situ skin environment.
