Botryococcus Terribilis Ethanol Extract Exerts Anti-Inflammatory Effects on Murine RAW264 Cells

The study investigated the anti-inflammatory effects of Botryococcus terribilis Ethanol Extract (BTEE) on murine RAW264 cells. BTEE significantly reduced nitric oxide production and the release of inflammatory cytokines, including Ccl2, Cox2, and Il6, while increasing the presence of anti-inflammatory mediators such as Pgc1β and Socs1. Gene expression changes included downregulation of genes associated with response to stimulus, signaling, locomotion, immune system process, and upregulation of genes related to the mitotic cell cycle. The study also identified AXL receptor tyrosine kinase (Axl) as a potential molecular target of BTEE's biological functions. The findings suggest that BTEE may have potential anti-inflammatory effects by regulating gene expression in cells. Further testing in human cell lines and in vivo is necessary.