Deletion Analysis of AGD1 Reveals Domains Crucial for Its Plasma Membrane Recruitment and Function in Root Hair Polarity

June 2017 in “ Journal of cell science ”

Chang Kyoo Yoo, Satoshi Naramoto, Jeffrey A. Sparks, Bibi Rafeiza Khan, Jin Nakashima, Hiroo Fukuda, Elison B. Blancaflor

root hair polarity AGD1 ACAP-type ARF-GAP Arabidopsis thaliana plasma membrane pleckstrin homology domain phosphoinositide-4-phosphate PI(4)P root hair growth PH domain

TLDR AGD1's PH domain is essential for its role in root hair growth and polarity.

The study investigated the role of AGD1, an ACAP-type ARF-GAP, in root hair polarity in Arabidopsis thaliana. AGD1-GFP localization varied with root hair growth phases, being prominent at the apical-most plasma membrane (PM) during slow growth phases and dissipating during rapid growth. Deletion analysis revealed that the pleckstrin homology (PH) domain was crucial for AGD1's PM localization, binding specifically to phosphoinositide-4-phosphate (PI(4)P). The study concluded that AGD1, through its PH domain, is recruited to the root hair PM by binding to PI(4)P, playing a role in regulating root hair growth and polarity.

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Research cited in this study

1 / 1 results

AGD1, A Class 1 ARF-GAP, Acts in Common Signaling Pathways with Phosphoinositide Metabolism and the Actin Cytoskeleton in Controlling Arabidopsis Root Hair Polarity

research AGD1, A Class 1 ARF-GAP, Acts in Common Signaling Pathways with Phosphoinositide Metabolism and the Actin Cytoskeleton in Controlling Arabidopsis Root Hair Polarity

56 citations, December 2011 in “The Plant Journal”

AGD1 is important for root hair development in Arabidopsis, working with phosphoinositide signaling and the actin cytoskeleton.

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research Deletion Analysis of AGD1 Reveals Domains Crucial for Its Plasma Membrane Recruitment and Function in Root Hair Polarity

11 citations, June 2017 in “Journal of cell science”

AGD1's PH domain is essential for its role in root hair growth and polarity.