4-Aminopyridine Promotes Accelerated Skin Wound Healing via Neurogenic-Mediator Expression

March 2022 in “ Research Square (Research Square) ”

Mashanipalya Jagadeeshaprasad, Prem Kumar Govindappa, Amanda M. Nelson, Mark David Noble, John C. Elfar

4-aminopyridine potassium channel blocker skin wound healing hair follicle neogenesis vimentin alpha-smooth muscle actin dermis neuronal regeneration axons S100+ Schwann cells SRY-Box Transcription Factor 10 transforming growth factor-β substance P nerve growth factor human keratinocytes Schwann cells 4-AP TGF-β NGF

TLDR 4-aminopyridine, a FDA-approved drug, speeds up skin wound healing and tissue regeneration.

The document reveals that 4-aminopyridine (4-AP), a potassium channel blocker approved by the FDA, significantly improves skin wound healing. This includes faster wound closure, restoration of normal skin structure, increased vascularization and reinnervation, and enhanced hair follicle neogenesis. The study found that 4-AP increased levels of vimentin and alpha-smooth muscle actin in the healed dermis, contributing to tissue regeneration. It also promoted neuronal regeneration, as evidenced by increased numbers of axons and S100+ Schwann cells, and elevated expression of SRY-Box Transcription Factor 10. The treatment also boosted levels of transforming growth factor-β, substance P, and nerve growth factor, all crucial for wound healing. In-vitro studies showed that 4-AP enhanced the proliferation and migration of human keratinocytes and Schwann cells, and improved cellular interactions to accelerate wound healing. Therefore, 4-AP enhances many key aspects of successful wound healing and presents a promising new approach to improve skin wound healing and tissue regeneration.

View this study on researchsquare.com →

Discuss this study in the Community →

Related Community Posts Join

6 / 1000+ results

community 4 months result topical min 5% Nw6

in Progress Pictures 9 upvotes 3 days ago

The user experienced good hair growth using topical minoxidil 5% and finasteride for 4 months but lost progress after switching to oral minoxidil and finasteride and shaving their head. They have returned to using topical treatments and are seeking advice on similar experiences.

community 4 months treatment with 5% minoxidil twice daily and 1 mg finasteride EOD.

in Is this regrowth? 38 upvotes 1 week ago

A 26-year-old is using 5% minoxidil twice daily and 1 mg finasteride every other day, along with biotin and collagen supplements, for hair regrowth. They apply minoxidil to the temples and crown and experienced initial shedding in the first month.

community 4 years of finasteride and 3 months of dutasteride

in Finasteride/Dutasteride 42 upvotes 2 weeks ago

A 23-year-old has been using finasteride 5mg daily since age 19 and added dutasteride 0.5mg twice a week to prevent hairline recession. They report no side effects and credit early treatment for maintaining their hair.

community 4 months finasteride, someone tell me what’s going on.

in Is this regrowth? 17 upvotes 2 weeks ago

The user has been using finasteride for 4 months and minoxidil for over a year, experiencing significant hair loss and thinning. Others suggest it might be a shedding phase, with some users sharing similar experiences and eventual regrowth, while others express skepticism about the effectiveness of the treatments.

community 4 months after HT and 3 months on every other day 1.25 mg fin + 2x daily topical min

in Progress Pictures 50 upvotes 3 weeks ago

The user had a hair transplant with 2000 grafts in the frontal and mid-scalp areas and has been using finasteride and minoxidil for 3 months. The crown area is expected to improve with continued treatment, and results are generally positive so far.

community 4 months update with minoxidil!

in Progress Pictures 70 upvotes 1 month ago

A user shared their 4-month progress using 5% topical minoxidil and 2 months of oral dutasteride for hair loss, experiencing significant shedding but also new hair growth. They reported side effects like increased body hair and temporary low libido, and are considering adding topical finasteride to their regimen.

Related Research

1 / 1 results

research Wound Healing and Skin Regeneration

359 citations, January 2015 in “Cold Spring Harbor Perspectives in Medicine”

Hair growth phase and certain genes can speed up wound healing, while an inflammatory mediator can slow down new hair growth after a wound. Understanding these factors can improve tissue regeneration during wound healing.