Three-Dimensional Histological Structures of the Human Dermis

The study aimed to generate 3D images of the human dermis using collagen/elastin-based imaging to better understand skin microstructures, which could aid in creating bioengineered skin. The research found that elastic fibers were less abundant near hair follicles compared to areas further away, while collagen fibers were evenly distributed. Both fiber types were less prevalent near the epidermis and hypodermis. The study provided detailed measurements of the acellular dermal matrix (ADM), including a porosity of 68.3%±5.8%, a mean pore diameter of 131.2±96.8 μm, and a wall thickness of 207.2±251.7 μm. This was the first time 3D histological structures of the skin were presented, potentially benefiting future skin engineering efforts.