Three-Dimensional Culture of Plucked Human Hair Follicles Inside the Collagen Gel Matrix

July 1990 in “ PubMed ”

S. Arase, Yasutaka Sadamoto, Susumu Katō, Kenji Fujie, Hideki Nakanishi, Kiyoshi Takeda

human hair follicles collagen gel matrix epithelial cells outer root sheath keratinocytes DNA-synthesizing cells outer root sheath cells

TLDR Human hair follicles can grow and form structures in a collagen gel, useful for studying hair cell growth.

The study explored the three-dimensional culture of plucked human hair follicles within a collagen gel matrix. Epithelial cells, likely outer root sheath keratinocytes, emerged from the outer root sheath 4 to 5 days after culturing and continued to grow into the gel, forming spike-like structures over the next 3 weeks. The number and size of these spikes varied among follicles. Autoradiography revealed numerous DNA-synthesizing cells in the outer cell layer of the enlarged outer root sheath and at the edges of the new spike-like structures. This culture method was deemed suitable for studying the growth, morphogenesis, and differentiation of outer root sheath cells in vitro.

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