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This study established an efficient short-term culture system for human hair follicle stem cells using human fibronectin and ROCK inhibitor Y-27632, enhancing cell proliferation and stemness for potential applications in hair follicle tissue engineering.

This study developed an Asian human sebocyte cell line from breast skin tissue that can be used for sebaceous gland biology investigations, showing that Y27632 may promote cell growth through EGFR-CRAF-MEK-ERK and AKT pathways.

This study found that the ROCK inhibitor Y-27632 maintains self-renewal and stemness of hair follicle stem cells in vitro, supporting their potential for tissue engineering applications.

This study found that Y27632 treatment facilitated the formation of core-shell-shaped hair follicle organoids, resulting in nearly 100% efficient hair shaft sprouting in vitro.

This study found that combining polybrene and a ROCK inhibitor can effectively expand human keratinocyte stem/progenitor cells carrying a transgene, aiding their use in regenerative medicine.